- July 14, 2026
Topic/Product:
CNS, Neurology
Disease Area:
Alzheimer's Disease
Sample Type:
Plasma
Background
The early and accurate detection of Alzheimer’s Disease (AD) remains a significant challenge, particularly with respect to blood-based biomarkers. While certain markers — such as brain-derived isoforms of Tau — have demonstrated promise for distinguishing AD from other dementias, there is a pressing need for accessible, cost-effective assays that combine multiple key markers with high sensitivity. To address this gap, we developed an ultrasensitive 5-plex AD panel using the novel NUcleic-acid Linked Immuno-Sandwich Assay (NULISA) technology1.
Our qPCR-based NULISA 5-plex AD assay targets five critical biomarkers: brain-derived pTau-217 (BD-pTau-217), NfL, GFAP, Ab42, and APOE4. The first four markers, which have been demonstrated to have strong associations with various phenotypes of AD, are detected quantitatively with ultra-high sensitivity. APOE4, an emerging new marker and the strongest generic risk factor for the late onset of AD, is assessed using a qualitative genotyping assay to distinguish carriers from non-carriers. Analytical performance was evaluated in plasma samples to assess detectability, spike-recovery, dilutional linearity, and parallelism. Assay correlations were established with both the NULISA single-plex assay and Neuro 220 Panel.
Authors:
Xiao-Jun Ma, Anna Lee, Prae Pongtornpipat, Thu Yoshimura, Pragya Mukherjee, Katie Cha, Lucas Zhen, Lucie Lee, Andrei Komarov, John Tenney, Henry Huang, Dwight Kuo, Jinwei Du, Kavita Tirumale, Zach Bent, Wei Feng, Yuling Luo
Alamar Biosciences, Fremont, CA
